产品订购信息：
英文名称  Anti-POMT1 

中文名称   蛋白甘露糖基转移酶1抗体规格 

别    名  POMT1_HUMAN; Protein O-mannosyl-transferase 1; Dolichyl-phosphate-mannose--protein mannosyltransferase 1.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow 

产品类型   一抗  

研究领域     细胞生物 免疫学 神经生物学 糖蛋白

蛋白分子量  predicted molecular weight: 85kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human POMT1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

蛋白甘露糖基转移酶1抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 POMT1 (Protein O mannosyl transferase 1) is a multipass membrane protein that is found in the endoplasmic reticulum. POMT1 catalyses the transfer of mannosyl residues to the hydroxyl groups of serine or threonine residues. Enzymatic activity is dependent on co expression of POMT1 with POMT2. Defects in the POMT1 gene are associated with Walker-Warburg syndrome (WWS), a congential muscular dystrophy that is associated with mental retardation and is usually lethal within the first few months of life. Other defects in the POMT1 gene result in limb girdle muscular dystrophy type 2K (LGMD2K), which is associated with mild mental retardation. Studies in Drosophila suggest that mutation of POMT1 alters the efficacy of synaptic transmission and a change in subunit composition of post synaptic glutamate receptors at the neuromuscular junction. Missense mutations in POMT1 have been associated with glioneuronal and glial brain tumours.

Function : Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.

Subunit : Interacts with POMT2 (Probable).

Subcellular Location : Endoplasmic reticulum membrane; Multi-pass membrane protein.

DISEASE : Muscular dystrophy-dystroglycanopathy congenital with mental retardation B1 (MDDGB1) [MIM:613155]: An autosomal recessive disorder characterized by congenital muscular dystrophy associated with mental retardation and mild structural brain abnormalities. Note=The disease is caused by mutations affecting the gene represented in this entry.

Muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A1 (MDDGA1) [MIM:236670]: An autosomal recessive disorder characterized by congenital muscular dystrophy associated with cobblestone lissencephaly and other brain anomalies, eye malformations, profound mental retardation, and death usually in the first years of life. Included diseases are the more severe Walker-Warburg syndrome and the slightly less severe muscle-eye-brain disease. Note=The disease is caused by mutations affecting the gene represented in this entry.

Muscular dystrophy-dystroglycanopathy limb-girdle C1 (MDDGC1) [MIM:609308]: An autosomal recessive degenerative myopathy associated with mild mental retardation without any obvious structural brain abnormality. An abnormal alpha-dystroglycan pattern in observed in the muscle. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the glycosyltransferase 39 family.

Contains 3 MIR domains.

Database links : UniProtKB/Swiss-Prot: Q9Y6A1.3

Anti-ATP2c1/Ca++ ATPase/FITC 荧光素标记钙离子ATP酶通道蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-GADD153/FITC 荧光素标记生长抑制DNA损伤基因153抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CLPTM1L/CRR9 顺氯氨铂耐药相关蛋白9抗体 规格 0.2ml

midnolin isoform Protein 2 中脑核仁蛋白2(抗原) 0.5mg

IGLK 英文名称： IV型己糖激酶抗体 0.2ml

Rhesus antibody Rh TRPM1 瞬时受体电位离子通道蛋白1抗体(M亚家族) 规格 0.2ml

Anti-GADD153/FITC 荧光素标记生长抑制DNA损伤基因153抗体IgGMulti-class antibodies规格： 0.2ml

Anti-IKB alpha(phospho S32 + S36)/FITC 荧光素标记磷酸化KB抑制蛋白α抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-PLK1 (Thr210)/FITC 荧光素标记磷酸化丝/苏氨酸蛋白激酶Plk1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ARHGAP20 Rho GTP酶激活蛋白20抗体 规格 0.2ml

Rabbit Anti-Mink IgG/PE-CY5 PE-CY5标记的兔抗水貂IgG 0.1ml

GADL1 英文名称： 谷酸脱羧酶样蛋白1抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-Mink IgG/RBITC 罗丹明标记的兔抗水貂IgG 规格 0.1ml

Anti-Phospho-PLK1 (Thr210)/FITC 荧光素标记磷酸化丝/苏氨酸蛋白激酶Plk1抗体IgGMulti-class antibodies规格： 0.2ml

NT-4 ELISA Kit 大鼠神经营养因子4Multi-class antibodies规格： 48T

Anti-B7-H1/PDL1/CD274 /FITC 荧光素标记程序性死亡配体1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh EURL/C21orf91 21号染色体开放阅读框91抗体 规格 0.2ml

PF-4/CXCL4 (Rabbit　Platelet Factor 4) ELISA Kit 兔子血小板因子4 96T

Phospho-MKK3(Ser218) + MKK6(Ser207) 英文名称： 磷酸化原活化蛋白激酶MKK3/6抗体 0.1ml

APAF1(CT) 英文名称： 凋亡蛋白活性因子-1抗体(C端) 0.1ml

Anti-B7-H1/PDL1/CD274 /FITC 荧光素标记程序性死亡配体1抗体IgGMulti-class antibodies规格： 0.2ml

615小鼠前瘤株;Fc

ART4 Others Cynomolgus 食蟹猴 ART4 人细胞裂解液 (阳性对照)

CM-M029小鼠肠平滑肌细胞完全培养基100mL

TE-11细胞，人细胞 人细胞,TJ905细胞 犬肾细胞;MDCK(NBL-2)

Calu-3(人) 5×106cells/瓶×2

CD40 Others Human 人 CD40 / TNFRSF5 人细胞裂解液 (阳性对照)

615小鼠前瘤株;Fc

ART4 Others Cynomolgus 食蟹猴 ART4 人细胞裂解液 (阳性对照)

CM-M029小鼠肠平滑肌细胞完全培养基100mL

TE-11细胞，人细胞 人细胞,TJ905细胞 犬肾细胞;MDCK(NBL-2)

Calu-3(人) 5×106cells/瓶×2

CD40 Others Human 人 CD40 / TNFRSF5 人细胞裂解液 (阳性对照)

蛋白甘露糖基转移酶1抗体规格 人表皮角质细胞-(HEK-a) ( 5×105 )

人肺粘液上皮样癌细胞;NCI-H292

大鼠骨髓间充质干细胞(BMSCs)

人细胞;CNE-2Z 大鼠肾上皮细胞完全培养基 100mL

NG108-15 大鼠小鼠杂合神经细胞

IL2RA Others Mouse 小鼠 IL2RA / CD25 人细胞裂解液 (阳性对照)

抗体的生物素化标记实验要点：

1. 蛋白甘露糖基转移酶1抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）蛋白甘露糖基转移酶1抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。