产品订购信息：
英文名称  Anti-Profilin 1 

中文名称   前纤维蛋白1抗体品牌 

别    名  Actin binding protein; PFN 1; Pfn; PFN1; Profilin I; Profilin1; ProfilinI; PROF1_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Horse 

产品类型   一抗  

研究领域     细胞生物 神经生物学 信号转导 干细胞 细胞周期蛋白 细胞分化 细胞类型标志物

蛋白分子量  predicted molecular weight: 15kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Profilin 1 C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

前纤维蛋白1抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Profilin 1 is a ubiquitous actin monomer-binding protein involved in actin polymerization in response to extracellular signals. Three profilin genes have been identified: profilin 1, 2, and 3. Profilin 1 is the most ubiquitous and abundant, and is highly expressed throughout development and adulthood inmost tissues including brain. Profilin 2 is the neuronal specific isoform and profilin 3 is a testis specific isoform. Profilins of eukaryotic cells are small (12-15 kDa) cytoplasmic proteins that bind to actin monomers, polyphosphoinositides and polymers of L-proline. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Deletion of profilin 1 gene leads to an embryonic lethal phenotype.

Function : Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

Subunit : Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with VASP. Interacts with HTT.

Subcellular Location : Cytoplasm, cytoskeleton.

Post-translational modifications : Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.

DISEASE : Amyotrophic lateral sclerosis 18 (ALS18) [MIM:614808]: A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the profilin family.

Database links : UniProtKB/Swiss-Prot: P07737.2

前纤维蛋白(profilin)是一种低分子量的肌动蛋白结合蛋白, 前纤维蛋白除了能与肌动蛋白结合之外,在分子间相互作用的复杂网络中还起到一个控制中心的作用。

Anti-Phospho-SHIP2 (Tyr1135)/FITC 荧光素标记磷酸化蛋白酪氨酸磷酸酶2抗体IgGMulti-class antibodies规格： 0.2ml

ART(Artemin)(抗原) Multi-class antibodies规格： 0.5mg

神经激肽 B抗体 Anti-NKB 0.2ml

SOX2 英文名称： 胚胎干细胞关键蛋白抗体 0.1ml

EPHB3/Eph receptor B3 英文名称： 内皮细胞受体蛋白酪酸激酶B3抗体 0.1ml

Rhesus antibody Rh Phospho-p57 Kip2 (Thr310) 磷酸化周期蛋白依赖激酶抑制因子1C抗体 规格 0.1ml

ART(Artemin)(抗原) Multi-class antibodies规格： 0.5mg

Anti-HRH4/GPCR105/FITC 荧光素标记组织胺H4受体抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Synapsin II /FITC 荧光素标记神经突触素2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ADK/AK 腺苷酸激酶抗体 规格 0.1ml

Rabbit Anti-Goat IgG/PE-Cy5.5 PE-Cy5.5标记的兔抗羊IgG 0.1ml

GABRA4 英文名称： G基A型受体α4/GABAA Rα4抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-chicken IgM/HRP 辣根过氧化物酶标记的兔抗鸡IgM 规格 0.1ml

Anti-Synapsin II /FITC 荧光素标记神经突触素2抗体IgGMulti-class antibodies规格： 0.2ml

IL-12 小鼠白介素-12Multi-class antibodies规格： 48T

Anti-phospho-MK1/MAPKK1(Ser298) 磷酸化原活化蛋白激酶激酶1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh MTMR8 肌管素相关蛋白8抗体 规格 0.2ml

F XII (Human coagulation factor XII ) ELISA Kit 人凝血因子Ⅻ 96T

Sialoadhesin 英文名称： 唾液酸结合性免疫球蛋白样凝集素1抗体 0.2ml

C6orf226 英文名称： 6号染色体开放阅读框226抗体 0.2ml

Anti-phospho-MK1/MAPKK1(Ser298) 磷酸化原活化蛋白激酶激酶1抗体Multi-class antibodies规格： 0.1ml

ColumbiaBloodAgarBase

DCLS agar Deoxycholate citrate lactose sucrose agar 1.10270.0500 MERCK默克 incubation media DCLS agar Deoxycholate citrate lactose sucrose agar 1.10270.0500 MERCK默克

脑—心浸萃液态培养基 100g 广泛用于霉菌、酵母、细菌的培养，包括营养要求较高的微生物的培养，特别用于饮用天然矿泉水和城...

SD大鼠骨髓间质干细胞成脂诱导分化培养基SDratbonemarrowmesenchymalstemcellsintotheculturemediuminduceddifferentiationoffat

UVM培养基 250g 用于李氏菌的增菌培养(MERCK方法)

BIGGY琼脂250g用于念珠菌的分离培养和计数

孟加拉红(虎红)培养基 1000(g) incubation media 孟加拉红(虎红)培养基 1000(g)

超级琼脂 Super Agar 250克 BR

消毒灭菌效果评价incubationmedia消毒灭菌效果评价

CCAgarSupplement

前纤维蛋白1抗体品牌 Fraser肉汤增菌液FB1配套试剂  规格：  2x5支  用途：  试剂A和试剂B各一支添加于225ml（026080）中配成FB1增菌液。

PALCAM琼脂冻干配套试剂  规格：  10支  用途：  每支添加于100ml（026070）中配成PALCAM琼脂。

李氏增菌液LB2冻干配套试剂  规格：  10支  用途：  每支添加于200ml（026040）中配成LB2增菌液。

李氏增菌液LB1冻干配套试剂  规格：  10支  用途：  每支添加于225ml（026040）中配成LB1增菌液。

抗体的生物素化标记实验要点：

1. 前纤维蛋白1抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）前纤维蛋白1抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。