抗体的生物素化标记实验要点：

1. 磷酸化P63抑制基因抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Phospho-p63 (Ser160/Ser162) 

中文名称   磷酸化P63抑制基因抗体品牌 

别    名  p63 (phospho S160/162); p63 (phospho Ser160/Ser162); p-p63 (Ser160/Ser162); AIS; Amplified in squamous cell carcinoma; B(p51A); B(p51B); p63 Alpha; Chronic ulcerative stomatitis protein; CUSP; DN p63 alpha 1; DNp63; EEC3; Keratinocyte transcription factor; Keratinocyte transcription factor KET; KET; LMS; NBP; OFC8; p40; p51; P51/P63; p53 like transcription factor; p53 related protein; p53-related protein p63; p53CP; p63; P73; p73H; p73L; RHS; SHFM4; TP53CP; TP53L; TP63; TP73; TP73L; Transformation related protein 63; Trp53rp1; Trp63; Tumor protein 63; tumor protein 63 kDa with strong homology to p53; Tumor protein p53-competing protein; Tumor protein p53-like; tumor protein p63; Tumor protein p73; tumor protein p73-like; P63_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Sheep 

产品类型   一抗 磷酸化抗体  

研究领域     细胞生物 免疫学 转录调节因子

蛋白分子量  predicted molecular weight: 77kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human p63 around the phosphorylation site of Ser160/Ser162 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化P63抑制基因抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq, Jul 2008].

Function : Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.

Subunit : Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L.

Subcellular Location : Nucleus.

Post-translational modifications : May be sumoylated.

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

DISEASE : Acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]: A form of ectodermal dysplasia. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontiaand loss of permanent teeth. ADULT syndrome differs significantly from EEC3 syndrome by the absence of facial clefting. Note=The disease is caused by mutations affecting the gene represented in this entry.

Ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]: An autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiform adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate. Note=The disease is caused by mutations affecting the gene represented in this entry.

Ectrodactyly, ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3) [MIM:604292]: A form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. It is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting. Note=The disease is caused by mutations affecting the gene represented in this entry.

Split-hand/foot malformation 4 (SHFM4) [MIM:605289]: A limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting. Note=The disease is caused by mutations affecting the gene represented in this entry.

Limb-mammary syndrome (LMS) [MIM:603543]: Characterized by ectrodactyly, cleft palate and mammary-gland abnormalities. Note=The disease is caused by mutations affecting the gene represented in this entry.

Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and

Ectodermal dysplasia, Rapp-Hodgkin type (EDRH) [MIM:129400]: A form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. Characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate. Note=The disease is caused by mutations affecting the gene represented in this entry.

Non-syndromic orofacial cleft 8 (OFC8) [MIM:129400]: A birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the p53 family.

Contains 1 SAM (sterile alpha motif) domain.

Database links : UniProtKB/Swiss-Prot: Q9H3D4.1

P63—也是抑制因子之一.p63基因是基因p53的同源性基因，主要表达上皮组织的基底细胞或复层上皮细胞，如皮肤、食道、宫颈外口、扁桃体、膀胱等，和、乳腺腺样结构的基层细胞。

抗体的鉴定：

1）磷酸化P63抑制基因抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-Sema4C/FITC 荧光素标记臂板蛋白4C抗体IgGMulti-class antibodies规格： 0.2ml

MMP-9(matrix metalloproteinase 9) 基质金属蛋白酶-9(抗原)Multi-class antibodies规格： 0.5mg

粘蛋白-1/上皮膜抗原抗体 Anti-mucin-1/Muc-1/CD227 antigen 0.1ml

SOX6 英文名称： 核转录因子SOX6抗体 0.2ml

Phospho-FRAP1 (Ser2448) 英文名称： 磷酸化雷帕霉素靶蛋白抗体 0.1ml

Rhesus antibody Rh Phospho-Paxillin (Tyr118) 磷酸化桩蛋白Paxillin抗体 规格 0.1ml

MMP-9(matrix metalloproteinase 9) 基质金属蛋白酶-9(抗原)Multi-class antibodies规格： 0.5mg

Anti-phospho-IkB beta (Ser23)/FITC 荧光素标记磷酸化KB抑制蛋白β抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Pokemon/FITC 荧光素标记兔抗蒙抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ARH/LDL receptor adaptor protein 受体衔接蛋白抗体 规格 0.2ml

Rabbit Anti-Mink IgG/PE PE标记的兔抗水貂IgG 0.1ml

GABPB2 英文名称： GA结合蛋白转录因子β/GABP-β1/GABP-β2抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-Mink IgG/PE-Cy5.5 PE-Cy5.5标记的兔抗水貂IgG 规格 0.1ml

Anti-Pokemon/FITC 荧光素标记兔抗蒙抗体IgGMulti-class antibodies规格： 0.2ml

PI3K 大鼠磷脂酰肌醇激酶Multi-class antibodies规格： 48T

Anti-EPEC/E.coli 肠致病性大肠杆菌抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh mucin 5B/Muc5B 粘蛋白-5B抗体 规格 0.2ml

PF-3(Human platelet factor 3) ELISA Kit 人血小板因子3 96T

SIM2 英文名称： 转录因子蛋白SIM2抗体 0.2ml

CSFV 英文名称： 猪瘟病毒抗体 0.1ml

Anti-EPEC/E.coli 肠致病性大肠杆菌抗体Multi-class antibodies规格： 0.2ml

L-酪酸营养琼脂基础28382250g用于蜡样芽孢杆菌的L-酪酸分解试验。

Dermasel 琼脂 (CM0539) Oxoid incubation media Dermasel 琼脂 (CM0539) Oxoid

酸土脂环芽孢杆菌 牛奶中青霉素的检测;用于益生菌类食品 支/瓶

PresenceAbsenceBroth

Mueller-HintonAgar

抗生素5号250g用于两性霉素B检定菌种培养

玫瑰红琼脂 250(g) incubation media 玫瑰红琼脂 250(g)

沙氏液体培养基 250g 用于真菌的增菌培养，还用于一次性使用卫生用品真菌定性检测

细菌琼脂粉250培养基原材料，培养基凝固剂incubationmedia细菌琼脂粉250培养基原材料，培养基凝固剂

WILKINS-CHALGREN琼脂 250g 用于厌氧菌的分离培养

磷酸化P63抑制基因抗体品牌 明胶培养基(营养明胶)  规格：  250g  用途：  供鉴别测定细菌液化明胶用

营养琼脂培养基NA  规格：  250g  用途：  供细菌总数测定、保存菌种及纯培养用，也可用于消毒效果测定。(GB)

伊红美蓝琼脂培养基(EMB)  规格：  250g  用途：  用于分离肠道致病菌特别是大肠菌群和粪大肠菌群。(GB、美国FDA、USP、ISO)

乳糖胆盐培养基（含中和剂）  规格：  250g  用途：  用于化妆品粪大肠菌群的测定。(化妆品卫生规范)