磷酸化3磷酸肌醇依赖性蛋白激酶1抗体费用 英文名称  Anti-Phospho-PDPK1(Ser410)

中文名称  磷酸化3磷酸肌醇依赖性蛋白激酶1抗体费用 

别    名 PDPK1(phospho S410); 3 phosphoinositide dependent protein kinase 1; hPDK 1; hPDK1; MGC20087 MGC35290; PDK 1; PDPK 1; PDPK1; PkB kinase; PkB kinase like gene 1; PkB like 1; PkB like; PRO0461; PDPK1_HUMAN.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat

产品类型   一抗 磷酸化抗体  

研究领域    神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 61kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human PDPK1 around the phosphorylation site of Ser410

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化3磷酸肌醇依赖性蛋白激酶1抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms.

Function : Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

Subunit : Interacts with NPRL2.

Subcellular Location : Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.

Post-translational modifications : Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.

Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.

Contains 1 PH domain.

Contains 1 protein kinase domain.

PDK1是蛋白激酶B(PKB)的上游激酶,通过与3,4,5三磷酸磷脂酰肌醇作用激活相邻的PKB分子.PDK1能够激活包含PKB在内的一系列的AGC激酶家族成员.从而调节,生长,扩散,生存,抗凋亡等诸多生理过程.

实验流程：

全、新、优、品、好四大特点：

磷酸化3磷酸肌醇依赖性蛋白激酶1抗体费用 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

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