中文名称  中间丝相关蛋白抗体说明书 

英文名称  Anti-Repetin

别    名 Intermediate filament associated protein; RPTN; RPTN_HUMAN.

产品属性：

中间丝相关蛋白抗体说明书 浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Pig, Cow, Horse

产品类型   一抗  

研究领域    细胞生物 信号转导 细胞周期蛋白 细胞分化

蛋白分子量  predicted molecular weight: 91kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Repetin

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

中间丝相关蛋白抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Repetin is a 784 amino acid secreted protein that belongs to the S100-fused protein family. Repetin is an extracellular epidermal matrix protein that is expressed in the epidermis and at high levels in eccrine sweat glands, the inner sheaths of hair roots and the filiform papilli of the tongue. A multifunctional epidermal matrix protein, repetin is involved in the cornified cell envelope formation. Containing two EF-hand domains, repetin’s recombinant N-terminal domain binds calcium reversibly. Repetin and trichohyalin may compensate for absent loricrin. The repetin gene contains three exons, two introns and spans at least 5.6 kb. The repetin gene is conserved in cow, mouse and rat, and maps to human chromosome 1q21.3.

Function : Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium.

Subcellular Location : Secreted; extracellular space; extracellular matrix.

Tissue Specificity : Repetin is a multifunctional epidermal matrix protein involved in the cornified cell envelope formation. It reversibly binds calcium. Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheat and in the filiform papilli of the tongue.

Post-translational modifications : Potential substrate of transglutaminase. Some arginines are probably converted.

Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Belongs to the S100-fused protein family.

Contains 2 EF-hand domains.

Database links : UniProtKB/Swiss-Prot: Q6XPR3.1

实验流程：

全、新、优、品、好四大特点：

中间丝相关蛋白抗体说明书 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

技术外包服务:

★中间丝相关蛋白抗体说明书 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

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