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★核酮糖1,5二磷酸羧化酶抗体品牌 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

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实验流程：

核酮糖1,5二磷酸羧化酶抗体品牌 英文名称  Anti-RUBISCO

中文名称  核酮糖1,5二磷酸羧化酶抗体品牌 

别    名 rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Arabidopsis Thaliana

产品类型   一抗  

研究领域    细胞生物 免疫学 信号转导

蛋白分子量  predicted molecular weight: 52kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from Arabidopsis thaliana RUBISCO

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

核酮糖1,5二磷酸羧化酶抗体品牌 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.

Sequence similarities: Belongs to the RuBisCO large chain family. Type I subfamily.

Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.

Subunit : Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers.

Subcellular Location : Plastid, chloroplast.

Similarity : Belongs to the RuBisCO large chain family. Type I subfamily.

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