抗体的生物素化标记实验要点：

1. 环指蛋白139抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-RNF139 

中文名称   环指蛋白139抗体费用 

别    名  HRCA1; RCA1; Ring finger protein 139; TRC8; RN139_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域     免疫学 信号转导

蛋白分子量  predicted molecular weight: 76kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RNF139 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

环指蛋白139抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Function : E3-ubiquitin ligase; acts as a negative regulator of the cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP/SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination. May function as a signaling receptor.

Subunit : Interacts with VHL. Interacts with MHC class I and HM13. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts with SREBF2 (via C-terminus domain). Interacts with SCAP; the interaction inhibits the interaction of SCAP with SEC24B and hampering the ER to Golgi transport of the SCAP/SREBP complex. Interacts with SEC24B. Interacts with INSIG1 and INSIG2. Interacts with EIF3F and EIF3H; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner.

Subcellular Location : Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue Specificity : Highly expressed in testis, placenta and adrenal gland. Moderate expression in heart, brain, liver, skeletal muscle and pancreas, and low expression in lung and kidney.

Post-translational modifications : Autoubiquitinated. Ubiquitination is induced by sterol and leads to ist degradation via the ubiquitin-proteasome pathway.

DISEASE : Defects in RNF139 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Note=A chromosomal aberration involving RNF139 has been found in a lymphoblastoid cell line established from a family with renal cell carcinoma and thyroid carcinoma. Translocation (3;8)(q14.2;q24.1) with FHIT. RNF139 is found to be fused to FHIT and disrupted within the sterol-sensing domain. In contrast, the FHIT coding region is maintained and expressed. Sporadic cases of renal carcinoma, where an acquired mutation in RNF139 results in the duplication of 12 nucleotides in the 5'-UTR, has also been identified.

Similarity : Contains 1 RING-type zinc finger.

抗体的鉴定：

1）环指蛋白139抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Irna(Human Immune RNA) ELISA Kit 人免疫核糖核酸Multi-class antibodies规格： 48T

Anti-phospho-FSCN1(Ser39) 磷酸化纤维束蛋白同源物1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Histone H3 (Di Methyl K4) 二甲基化组蛋白H3K4抗体 规格 0.1ml

SRP(Human signal recognization particle antibody) ELISA Kit 人抗信号识别颗粒抗体 96T

recPEP 英文名称： 重组猪病毒结构蛋白抗体 0.1ml

COMMD3 英文名称： 铜代谢结构域蛋白3抗体 0.2ml

Anti-phospho-FSCN1(Ser39) 磷酸化纤维束蛋白同源物1抗体Multi-class antibodies规格： 0.1ml

Anti-RRM1/FITC 荧光素标记抗核糖核苷酸还原酶1抗体IgGMulti-class antibodies规格： 0.2ml

IGF-II( Insulin-like Growth Factor-II ) 样生长因子-II(抗原)Multi-class antibodies规格： 0.5mg

黑素瘤抗原-1抗体 Anti-MAGE-1/HLA-A1 protein 0.2ml

SLC27A2 英文名称： 长链脂肪酸辅酶A连接酶抗体 0.1ml

FSTL5 英文名称： 卵泡抑素相关蛋白5抗体 0.2ml

Rhesus antibody Rh Phospho-Pin1 (Ser16) 磷酸化肽基脯氨酞顺反异构酶Pinl抗体 规格 0.1ml

IGF-II( Insulin-like Growth Factor-II ) 样生长因子-II(抗原)Multi-class antibodies规格： 0.5mg

LIF 大鼠抑制因子Multi-class antibodies规格： 48T

Anti-ECP 嗜酸性粒细胞阳离子蛋白抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh MYPT1/MBS 肌球蛋酸酶抗体 规格 0.1ml

FPA(Human fibrinopeptide A) ELISA Kit 人血纤肽/纤维蛋白肽A 96T

SOX1 英文名称： 核转录因子抗体 0.2ml

CCL21 英文名称： 淋巴细胞趋化因子CCL21抗体 0.1ml

Anti-ECP 嗜酸性粒细胞阳离子蛋白抗体Multi-class antibodies规格： 0.2ml

溶菌酶5g炭疽杆菌检测用配套试剂

FRASER Listeria Selective Enrichment Broth( base) 1.10398.0500 MERCK默克 incubation media FRASER Listeria Selective Enrichment Broth( base) 1.10398.0500 MERCK默克

改良EC新生霉素增菌肉汤基础(mEC+n) 250g 用于致病性大肠杆菌O157:H7的增菌培养(GB/T 4789.36-2008, SN/T 1827-2006和SN/T0973-2000)。

兔骨髓间质干细胞成软骨诱导分化完全培养基Rabbitbonemarrowmesenchymalstemcellsintochondrocytesinduceddifferentiationofcompletemedium

UBA培养基 250g 用于啤酒中厌氧菌检测

芽孢杆菌培养基250g用于芽孢杆菌平板计数

李斯特菌鉴定显色培养基 用于将李斯特菌鉴定到种 250ml incubation media 李斯特菌鉴定显色培养基 用于将李斯特菌鉴定到种 250ml

类干酪乳杆菌类干酪亚种 支/瓶

LST发酵管(含小倒管)20支/包用于大肠菌群、大肠杆菌的测定

LactoseBileBroth

环指蛋白139抗体费用 1%NaCl甘露糖  规格：  20支/盒  用途：  用于鉴别耐的弧菌利用糖源的能力。（鉴定霍乱弧菌）

葡萄糖磷酸盐胨水生化鉴定管  规格：  20支/盒  用途：  细菌生化鉴定

1%NaCl甘露醇生化鉴定管  规格：  20支/盒  用途：  用于鉴别耐的弧菌利用糖源的能力。（鉴定霍乱弧菌）

蛋白胨水生化鉴定管  规格：  20支/盒  用途：  细菌生化鉴定