产品订购信息：
英文名称  Anti-phospho-ROCK1(Thr455/Ser456) 

中文名称   磷酸化Rho相关蛋白激酶1抗体费用 

别    名  p-ROCK1(Thr455/Ser456); p160 ROCK1; p160ROCK; Renal carcinoma antigen NY REN 35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; ROCK1_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Horse, Rabbit 

产品类型   一抗 磷酸化抗体  

研究领域     信号转导 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 158kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthesised phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455/Ser456 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化Rho相关蛋白激酶1抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq].

Function : Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation.

Subunit : Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.

Subcellular Location : Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.

Tissue Specificity : Detected in blood platelets.

Post-translational modifications : Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR.

Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PH domain.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 protein kinase domain.

Contains 1 REM (Hr1) repeat.

Database links :

UniProtKB/Swiss-Prot: Q13464.1

Entrez Gene: 6093 Human

Entrez Gene: 19877 Mouse

Entrez Gene: 81762 Rat

Omim: 601702 Human

SwissProt: Q8MIT6 Cow

SwissProt: Q13464 Human

SwissProt: P70335 Mouse

SwissProt: Q63644 Rat

ROCK1也是丝/苏氨基酸激酶的一种，可调节细胞因子，促进和肌动蛋白的形成,因此又称：含有卷曲螺旋的蛋白激酶。目前主要用于浸润、转移方面的研究。

Anti-TSP-1/THBS1/FITC 荧光素标记血小板反应蛋白/凝血酶敏感蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Trk B 神经生长因子受体的一种(抗原)Multi-class antibodies规格： 0.5mg

ZCWCC1抗体 Anti-ZCWCC1 0.2ml

Stanniocalcin 1 英文名称： 斯钙素抗体 0.1ml

Exportin 1/CRM1 英文名称： 染色体区域稳定蛋白/核输出蛋白1抗体 0.1ml

Rhesus antibody Rh Phospho-IKK alpha/beta (Ser176/Ser180) 磷酸化KB抑制蛋白激酶α/β抗体 规格 0.1ml

Trk B 神经生长因子受体的一种(抗原)Multi-class antibodies规格： 0.5mg

Anti-PPAR alpha/FITC 荧光素标记α型-过氧化酶活化增生受体抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-Bovine IgG Whole serum 兔抗IgG抗血清Multi-class antibodies规格： 1ml

干扰素-γ抗体 Anti-IFN-γAnti-mouse、rat 0.1ml

Goat Anti-Mouse IgG 羊抗小鼠IgG 1mg

Fragilis 英文名称： 干扰素诱导蛋白15抗体 0.2ml

Rhesus antibody Rh phospho-RAD51(Thr310) 磷酸化Rad51抗体 规格 0.1ml

Rabbit Anti-Bovine IgG Whole serum 兔抗IgG抗血清Multi-class antibodies规格： 1ml

PTN(Human pleiotrophin) ELISA Kit 人多效生长因子Multi-class antibodies规格： 48T

Anti-GAD65 谷氨酸脱羧酶-65抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Mouse Anti-human IgG/Cy5 Cy5标记的小鼠抗人IgG 规格 0.1ml

Alpha1 Beta-GP(Human Alpha1 Beta glycoprotein) ELISA Kit 人α1β糖蛋白 96T

sp-selectin 英文名称： 可溶性P选择素抗体 0.1ml

CELSR2 英文名称： 表皮生长因子样蛋白2抗体 0.1ml

Anti-GAD65 谷氨酸脱羧酶-65抗体Multi-class antibodies规格： 0.1ml

胰酪胨大豆肉汤24052250g用于金黄色葡萄球菌的多管发酵法测定及增菌培养(GB/T4789.08)。

H Medium Broth 培养基 100g incubation media H Medium Broth 培养基 100g

雪白根霉 检测用菌 支/瓶

营养肉汤(GB/T20944.7标准)250g一般细菌培养，转种，复壮，增菌等，也可用于消毒效果检测

营养琼脂 250g 细菌总数测定，细菌传代培养

NystatinTestMedium

抗生素检定培养基8号 250(g) incubation media 抗生素检定培养基8号 250(g)

TerrificBroth 500g 用于提高大肠杆菌中质粒DNA的成品得率Terrific broth is used with glycerol in cultivating rec...

去氧胆酸于incubationmedia去氧胆酸于

TSCAgarBase

磷酸化Rho相关蛋白激酶1抗体费用 规格：  微生物快速检测计数卡  用途：

高敏感度大肠菌群计数板  规格：  25片/袋  用途：  用于大肠菌群计数，24小时可确定大肠菌群数

大肠杆菌计数板  规格：  25片/袋  用途：  可同时进行大肠杆菌和大肠菌群计数，24-48小时可确认大肠杆菌。

菌落总数计数板  规格：  50片/袋  用途：  菌落总数检验

抗体的生物素化标记实验要点：

1. 磷酸化Rho相关蛋白激酶1抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）磷酸化Rho相关蛋白激酶1抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。