抗体的生物素化标记实验要点：

1. 维甲酸诱导蛋白6抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-STRA6 

中文名称   维甲酸诱导蛋白6抗体品牌 

别    名  Stimulated by retinoic acid gene 6 protein homolog; STRA6_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Horse, Rabbit 

产品类型   一抗  

研究领域     细胞生物 信号转导 细胞类型标志物

蛋白分子量  predicted molecular weight: 74kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from Human STRA6 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

维甲酸诱导蛋白6抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 STRA6 is a 667 amino acid, multi-pass cell membrane protein. Stra6 functions as a cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). Ultimately increasing cellular retinol uptake from the retinol-RBP complex, Stra6 removes retinol from RBP/RPB4 and transports it across the plasma membrane, where it is metabolized. Stra6 is broadly expressed, with 4 named isoforms that exist as a result of alternative splicing events. Mutations in the gene encoding Stra6 cause Matthew-Wood Syndrome, also known as Spear Syndrome. This syndrome is characterized by anophtalmia, mild facial dysmorphism and malformations of the heart, lung and diaphragm. The Stra6 gene maps to chromosome 15q24.1.

Function : Stra6 functions as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). Stra6 is expressed in the extraembryonic endoderm and expression is also known to be induced by Wnt1. Defects in STRA6 are known to cause of syndromic microphthalmia type 9 (MCOPS9) also known as clinical anophthalmia with mild facial dysmorphism and variable malformations of the lung, heart, and diaphragm or anophthalmia/microphthalmia and pulmonary hypoplasia or Spear syndrome or Matthew-Wood syndrome or pulmonary agenesis, microphthalmia, and diaphragmatic defect.

Subcellular Location : Cell membrane; multi-pass membrane protein.

Tissue Specificity : Broad expression. In adult eye expressed in sclera, retina, retinal pigment epithelium, and trabecular meshwork but not in choroid and iris.

DISEASE : Defects in STRA6 are the cause of microphthalmia syndromic type 9 (MCOPS9) [MIM:601186]; also called Matthew-Wood syndrome or Spear syndrome. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS9 is a rare clinical entity including as main characteristics anophthalmia or severe microphthalmia, and pulmonary hypoplasia or aplasia.

Note=Mutations in STRA6 may be a cause of isolated colobomatous microphthalmia, a disorder of the eye characterized by an abnormally small ocular globe.

抗体的鉴定：

1）维甲酸诱导蛋白6抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

NKA(Neurokinin A) 神经激肽A抗原Multi-class antibodies规格： 0.5mg

Anti-APP/ABPP 淀粉样肽前体蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Phospho-ATP1A1(Tyr10) 磷酸化ATP酶蛋白a1抗体 规格 0.1ml

山羊抗兔 IgG(H+L)/AP 0.1ml 美国Jackson公司分装

V5 tag 英文名称： V5 tag标签抗体 0.1ml

DKC1 英文名称： 核仁蛋白NAP57抗体 0.2ml

Anti-APP/ABPP 淀粉样肽前体蛋白抗体Multi-class antibodies规格： 0.1ml

Anti-phospho-c-Jun (Ser243) /FITC 荧光素标记磷酸化原癌基因c-Jun抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-chicken IgM 兔抗鸡IgM (亲和层析纯化)Multi-class antibodies规格： 1mg

胃泌素抑制肽抗体 Anti-GIP 0.2ml

Goat Anti-Bovine IgG/Cy3 Cy3标记的羊抗IgG 0.1ml

FHL2 英文名称： 相互作用蛋白FHL2抗体 0.1ml

Rhesus antibody Rh phospho-SIRT6(Ser338) 磷酸化沉默调节相关蛋白6抗体 规格 0.1ml

Rabbit Anti-chicken IgM 兔抗鸡IgM (亲和层析纯化)Multi-class antibodies规格： 1mg

5HT1E Receptor 英文名称： 5-羟色胺受体1E抗体 0.2ml

EMR1 英文名称： 表皮生长因子样激素受体1 0.2ml

腱糖蛋白-C抗体 anti-Tn-C 0.2ml

Anti-TNF- Alpha /HRP 辣根过氧化物酶标记鼠抗人坏死因子-α单克隆抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-JunB (Thr102/Thr104) 磷酸化活化蛋白激酶B抗体 规格 0.2ml

PLGF (Placenta growth factor) 胎盘生长因子(多肽抗原)Multi-class antibodies规格： 0.5mg

786-O [786-0], 人肾透明腺癌细胞

NRK 大鼠肾细胞

GPA33 Others Human 人 GPA33 / Glycoprotein-A33 人细胞裂解液 (阳性对照)

非洲绿猴肾细胞;Vero E6

视网膜微内皮细胞Many types of cells包装：5 × 105次方(1ml)

人B细胞;RAMOS(RA.1) 大鼠*星状细胞完全培养基 100mL

A-375 [A375], 人恶性黑素瘤细胞株

NINJ1 Others Rat 大鼠 Ninjurin-1 / NINJ1 人细胞裂解液 (阳性对照)

大鼠脑平滑肌细胞完全培养基 100mL

B95-8绒猴EBV转化的白细胞 B95-8 EBV conversion of leukocyte marmoset RPMI-1640(GIBCO)+10%FBS

IFNA13 Protein Mouse 重组小鼠 IFNA13 / Ierferon alpha-13 蛋白 (Fc 标签)

MFC-GFP(小鼠前细胞(绿色荧光蛋白标记)) 5×106cells/瓶×2 CTLL-2(T细胞)

维甲酸诱导蛋白6抗体品牌 786-O [786-0], 人肾透明腺癌细胞

NRK 大鼠肾细胞

GPA33 Others Human 人 GPA33 / Glycoprotein-A33 人细胞裂解液 (阳性对照)

非洲绿猴肾细胞;Vero E6

视网膜微内皮细胞Many types of cells包装：5 × 105次方(1ml)

人B细胞;RAMOS(RA.1) 大鼠*星状细胞完全培养基 100mL