产品订购信息：
英文名称  Anti-SRPX2 

中文名称   SRPX2蛋白抗体说明书 

别    名  SRPX2 is a 465 amino acid secreted protein expressed in neurons of the brain, including the rolandic area. It has been suggested that SRPX2 enhances cell motility, migration and adhesion through FAK signaling in gastric and other cancer cells. Localized to the cytoplasm, SRPX2 is a ligand for uPAR (urokinase plasminogen activator), a receptor that is a crucial component of the extracellular plasminogen proteolysis system. SRPX2 may be responsible for rolandic seizures (RSs) associated with oral and speech dyspraxia and mental retardation (MR). The involvement of SRPX2 in these disorders suggests an important role for SRPX2 in the perisylvian region critical for language and cognitive development; SRPX2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat 

产品类型   一抗  

研究领域     神经生物学

蛋白分子量  predicted molecular weight: 50kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SRPX2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

SRPX2蛋白抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SRPX2 is a 465 amino acid secreted protein expressed in neurons of the brain, including the rolandic area. It has been suggested that SRPX2 enhances cell motility, migration and adhesion through FAK signaling in gastric and other cancer cells. Localized to the cytoplasm, SRPX2 is a ligand for uPAR (urokinase plasminogen activator), a receptor that is a crucial component of the extracellular plasminogen proteolysis system. SRPX2 may be responsible for rolandic seizures (RSs) associated with oral and speech dyspraxia and mental retardation (MR). The involvement of SRPX2 in these disorders suggests an important role for SRPX2 in the perisylvian region critical for language and cognitive development.

Function : Acts as a ligand for the urokinase plasminogen activator surface receptor. Plays a role in angiogenesis by inducing endothelial cell migration and the formation of vascular network (cords). Involved in cellular migration and adhesion in cancer cells. Increases the phosphorylation levels of FAK. May have a role in the perisylvian region, critical for language and cognitive development.

Subunit : Interacts with ADAMTS4, CTSB and PLAUR. Interacts with PLAUR (via the UPAR/Ly6 domains).

Subcellular Location : Cytoplasm. Secreted.

Tissue Specificity : Expressed in neurons of the rolandic area of the brain (at protein level). Highly expressed in the brain, placenta, lung, trachea, uterus and adrenal gland. Weakly expressed in the peripheral blood, brain and bone marrow. Expressed in numerous cancer cell lines.

DISEASE : Defects in SRPX2 are a cause of bilateral perisylvian polymicrogyria (BPP) [MIM:300388]. BPP is the most common form of polymicrogyria, a malformation of the cortex, in which the brain surface is irregular and the normal gyral pattern replaced by multiple small, partly fused, gyri separated by shallow sulci. BPP results in mild mental retardation, epilepsy and pseudobulbar palsy, causing difficulties with expressive speech and feeding.

Defects in SRPX2 are a cause of rolandic epilepsy with speech dyspraxia and mental retardation X-linked (RESDX) [MIM:300643]. A condition characterized by the association of rolandic seizures with oral and speech dyspraxia, and mental retardation. Rolandic occur during a period of significant brain maturation. During this time, dysfunction of neural network activities such as focal discharges may be associated with specific developmental disabilities resulting in specific cognitive impairments of language, visuo-spatial abilities or attention.

Similarity : Contains 1 HYR domain.

Contains 3 Sushi (CCP/SCR) domains.

Database links : UniProtKB/Swiss-Prot: O60687.1

Anti-Phospho-Tuberin/TSC2 (Ser939) /FITC 荧光素标记磷酸化马铃薯球蛋白(性硬化)抗体IgGMulti-class antibodies规格： 0.2ml

NAIF1 protein( CB12-327) 核凋亡诱导因子蛋白1(多肽)Multi-class antibodies规格： 0.5mg

抗原抗体 Anti-CA125 0.1ml

SLC*8 英文名称： 转运体蛋白8抗体 0.1ml

EGF 英文名称： 表皮生长因子抗体 0.1ml

Rhesus antibody Rh phospho-HSP70(Tyr41) 磷酸化热休克蛋白70抗体 规格 0.1ml

NAIF1 protein( CB12-327) 核凋亡诱导因子蛋白1(多肽)Multi-class antibodies规格： 0.5mg

Anti-phospho-c-Raf (Ser296) /FITC 荧光素标记磷酸化原癌基因c-Raf抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-Biotin 兔抗生物素抗体IgG (亲和层析纯化)Multi-class antibodies规格： 1mg

GDNF家族受体α-4抗体 Anti-GFRA4 0.2ml

Rabbit Anti-chicken IgG/Alexa Fluor 350 Alexa Fluor 350标记的兔抗鸡IgG 0.1ml

FXR2 英文名称： 脆性X相关蛋白样2抗体 0.2ml

Rhesus antibody Rh Phospho-Smad1/5(Ser463/465) 磷酸化细胞信号转导分子Smad-1/5抗体 规格 0.1ml

Rabbit Anti-Biotin 兔抗生物素抗体IgG (亲和层析纯化)Multi-class antibodies规格： 1mg

proCNP/NPPC (pro-natriuretic peptide) 促尿排泄肽前体C抗原Multi-class antibodies规格： 0.5mg

Anti-AQP7 水通道蛋白-7抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-ATG4C(Ser166) 磷酸化自噬相关蛋白4C抗体 规格 0.1ml

山羊抗小鼠 IgG(H+L)/HRP 0.1ml 美国Jackson公司分装

Uromodulin 英文名称： TH糖蛋白抗体 0.2ml

DNA polymerase alpha 英文名称： DNA聚合酶α抗体 0.2ml

Anti-AQP7 水通道蛋白-7抗体Multi-class antibodies规格： 0.2ml

CF Protein Mouse 重组小鼠 CF / Ciliary Neuroophic Factor 蛋白 (His 标签)

小鼠肠粘膜上皮细胞完全培养基 100mL

SELP Others Human 人 SELP / selectin P / P-selectin 人细胞裂解液 (阳性对照)

3D4/21猪肺泡巨噬细胞 Porcine alveolar macrophage 3D4/21 1640+10% FBS+0.1mM NEAA+1mM 酸

CL-0183PA317(小鼠成纤维细胞)5×106cells/瓶×2

RBL-1(大鼠嗜碱性粒细胞性细胞) 5×106cells/瓶×2 PC-3(人细胞)

CM-M125小鼠牙细胞完全培养基100mL

IGSF8 Others Mouse 小鼠 PGRL / IGSF8 人细胞裂解液 (阳性对照)

小鼠肾实质细胞完全培养基 100mL

P19小鼠 P19 mouse teratoma cells a-MEM(肌醇 43.2mg/l,叶酸 8.82mg/l)+7.5%BCS+2.5%FBS

IL12A Protein Cynomolgus 重组食蟹猴 / 恒河猴 IL12A / NKSF1 蛋白 (Fc 标签)

SW 1990(人*癌细胞) 5×106cells/瓶×2 NCL-H460(非小细胞)

SRPX2蛋白抗体说明书 CF Protein Mouse 重组小鼠 CF / Ciliary Neuroophic Factor 蛋白 (His 标签)

小鼠肠粘膜上皮细胞完全培养基 100mL

SELP Others Human 人 SELP / selectin P / P-selectin 人细胞裂解液 (阳性对照)

3D4/21猪肺泡巨噬细胞 Porcine alveolar macrophage 3D4/21 1640+10% FBS+0.1mM NEAA+1mM 酸

CL-0183PA317(小鼠成纤维细胞)5×106cells/瓶×2

RBL-1(大鼠嗜碱性粒细胞性细胞) 5×106cells/瓶×2 PC-3(人细胞)

抗体的生物素化标记实验要点：

1. SRPX2蛋白抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）SRPX2蛋白抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。