产品订购信息：
英文名称  Anti-UCP-3 

中文名称   线粒体脱偶连蛋白3抗体说明书 

别    名  Uncoupling Protein-3; UCP3; UCP 3; mitochondrial uncoupling protein 3; Solute carrier family 25 member 9; SLC25A9; UCP3_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat 

产品类型   一抗  

研究领域     细胞生物 神经生物学 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 34kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human UCP-3 C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

线粒体脱偶连蛋白3抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The uncoupling proteins (UCPs) are found in the inner mitochondrial membranes and belong to a family of mitochondrial anion transport proteins that include the phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP 1 is abundantly expressed in brown adipose tissue of mammals. UCP 1 allows proton re-entry into the mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is thought that UCP 2 and 3 also dissipate the mitochondrial proton gradient produced by the respiratory chain in cells and tissues within which they are expressed. Studies have shown UCP 2 to be expressed by a variety of tissues. UCP 3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in heart and smooth muscle, and is absent in liver and kidney.

Function : UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.

Subcellular Location : mitochondrial.Mitochondrion inner membrane; Multi-pass membrane protein.

Tissue Specificity : Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.

DISEASE : Obesity (OBESITY) [MIM:601665]: A condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Note=The gene represented in this entry may be involved in disease pathogenesis.

Similarity : Belongs to the mitochondrial carrier family.

Database links : UniProtKB/Swiss-Prot: P55916.1

IL-32 ELISA Kit 大鼠白介素32Multi-class antibodies规格： 48T

Anti-phospho-Synapsin I (Ser9) 磷酸化神经突触素1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Goat Anti-Guinea pig IgG/Alexa Fluor 488 Alexa Fluor 488标记的羊抗豚鼠IgG 规格 0.1ml

FSA(Human fetal sulfoslycoprotein antigen) ELISA Kit 人胚胎性硫糖蛋白抗原 96T

NCE2 英文名称： 泛素结合酶E2F抗体 0.2ml

BTR1 英文名称： 钙粘蛋白相关蛋白受体BTR1抗体 0.1ml

Anti-phospho-Synapsin I (Ser9) 磷酸化神经突触素1抗体Multi-class antibodies规格： 0.1ml

Anti-GFAP/FITC 荧光素标记胶质纤维酸性蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Nur77/GFRP/FITC 荧光素标记孤儿核受体蛋白Nur77抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BMP4 骨形态发生蛋白4抗体 规格 0.1ml

MMP-7(Matrilysin/matrix metalloproteinases-7) 基质金属蛋白酶-7(抗原) 0.5mg

HSP60 英文名称： 热休克蛋白-60抗体 0.1ml

Rhesus antibody Rh RDH11 视黄醇脱氢酶11抗体(病毒核心蛋白) 规格 0.2ml

Anti-Nur77/GFRP/FITC 荧光素标记孤儿核受体蛋白Nur77抗体IgGMulti-class antibodies规格： 0.2ml

GAG(Human glycosaminoglycan) ELISA Kit 人糖胺聚糖Multi-class antibodies规格： 48T

Anti-Morphine *抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh KCNE2 离子通道蛋白家族成员2抗体 规格 0.2ml

Resistin ELISA Kit 大鼠抵抗素 96T

Phospho-PDGFRA(Tyr572)+PDGFRB(Tyr579) 英文名称： 磷酸化血小板源性生长因子受体α 0.1ml

CCDC11 英文名称： 卷曲螺旋结构域蛋白11抗体 0.2ml

Anti-Morphine *抗体Multi-class antibodies规格： 0.2ml

DDR2 Others Rat 大鼠 DDR2 Kinase / CD167b 人细胞裂解液 (阳性对照)

非洲绿猴肾细胞系/IgRCD4+;VERO/IgRCD4+

人肺大内皮细胞完全培养基 100mL

SK-MEL-1细胞，人皮肤细胞 德氏乳杆菌乳酸亚种 人十二脂肠腺癌;HuTu-80

ANGPTL7 Protein Canine 重组狗 ANGPTL7 / Angiopoietin-like 7 蛋白 (Fc 标签)

KG-1(人) 5×106cells/瓶×2 大鼠 PD-L1 / B7-H1 / CD274 人细胞裂解液 (阳性对照)

CL-0283Ishikawa(人细胞)5×106cells/瓶×2

PLK1 Others Human 人 PLK1 / PLK-1 杆状病毒-昆虫细胞裂解液 (阳性对照)

人小梁网细胞cDNAHTMC cDNA

PT-67细胞，病毒转染小鼠细胞 人肺巨细胞癌(PG)的高转移亚系,PG-BE1细胞 肠微内皮细胞Many types of cells包装：5 × 105次方(1ml)

犬肾细胞;MDCK(NBL-2)

TACSTD2 Others Human 人 OP2 / TACSTD2 人细胞裂解液 (阳性对照)

线粒体脱偶连蛋白3抗体说明书 CL-0282HO-8910PM(人高转移细胞)5×106cells/瓶×2

TNFSF11 Others Mouse 小鼠 RANKL / OPGL / TNFSF11 / CD254 人细胞裂解液 (阳性对照)

小鼠颌下腺上皮细胞完全培养基 100mL

L W-3A小鼠皮下结缔组织细胞 L W-3A mice subcutaneous connective tissue cells DMEM培养基+10%FBS

IL1A Protein Mouse 重组小鼠 IL-1 alpha / IL1A / IL1F1 蛋白

P3X63Ag8.653(小鼠细胞) 5×106cells/瓶×2 L6(大鼠成肌细胞)

抗体的生物素化标记实验要点：

1. 线粒体脱偶连蛋白3抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）线粒体脱偶连蛋白3抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。