中文名称  组蛋白甲基转移酶SMYD3抗体说明书 

英文名称  Anti-SMYD3

别    名 SET and MYND domain containing 3; SET and MYND domain containing protein 3; SMYD 3; SMYD3 protein; Zinc finger MYND domain containing 1; Zinc finger MYND domain containing protein 1; Zinc finger protein subfamily 3A MYND domain containing 1; ZMYND 1; ZMYND1; ZNFN3A1; SMYD3_HUMAN.

产品属性：

组蛋白甲基转移酶SMYD3抗体说明书 浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep

产品类型   一抗  

研究领域     免疫学 染色质和核信号 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 49kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SMYD3

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

组蛋白甲基转移酶SMYD3抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SMYD3 is a gene that is over-expressed in the majority of colorectal carcinomas and hepatocellular carcinomas. SMYD3 forms a complex with RNA polymerase II through an interaction with the RNA helicase HELZ and transactivates a set of genes that included oncogenes, homeobox genes and genes associated with cell-cycle regulation. SMYD3 binds to a motif, 5'-CCCTCC-3', present in the promoter region of downstream genes such as Nkx2.8. The SET domain of SMYD3 shows histone H3-lysine 4 (H3-K4)-specific methyltransferase activity, which is enhanced in the presence of the heat-shock protein HSP90A.

Function : Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.

Subunit : Interacts with HSPCA. Interacts with HELZ. Interacts with POLR2A; the interaction may be indirect and may be mediated by HELZ.

Subcellular Location : Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.

Tissue Specificity : Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.

Similarity : Belongs to the histone-lysine methyltransferase family.

Contains 1 MYND-type zinc finger.

Contains 1 SET domain.

Database links : UniProtKB/Swiss-Prot: Q9H7B4.4

实验流程：

全、新、优、品、好四大特点：

组蛋白甲基转移酶SMYD3抗体说明书 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

技术外包服务:

★组蛋白甲基转移酶SMYD3抗体说明书 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

正在热销的相关产品：

PAX8(Paired box gene 8) 配对盒基因8抗原Multi-class antibodies规格： 0.5mg

Anti-Phospho-ULK1 (Ser467) 磷酸化自噬相关蛋白1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-APBB1 (Ser175) 磷酸化铁蛋白Fe65抗体 规格 0.1ml

PSAP 浓缩液 0.1ml 进口分装

Ube2H 英文名称： 泛素激活酶2H抗体 0.2ml

DCAKD 英文名称： 脱磷酸辅酶A结构域蛋白抗体 0.2ml

Anti-Phospho-ULK1 (Ser467) 磷酸化自噬相关蛋白1抗体Multi-class antibodies规格： 0.1ml

Anti-CK19/Gold 胶体金离子标记细胞角蛋白19抗体IgGMulti-class antibodies规格： 0.2ml

Anti-IRAK4/FITC 荧光素标记白介素-1受体相关激酶4抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CCL24 嗜酸粒细胞趋化蛋白24抗体 规格 0.2ml

IL-16(Interleukin-16) 白介素16抗原 0.5mg

HPV16 E7 英文名称： 人瘤病毒16型E7抗体 0.1ml

Rhesus antibody Rh SPAG8 相关抗原8抗体 规格 0.2ml

Anti-IRAK4/FITC 荧光素标记白介素-1受体相关激酶4抗体IgGMulti-class antibodies规格： 0.2ml

TH(Human tyrosine hydroxylase) ELISA Kit 人酪氨酸羟化酶Multi-class antibodies规格： 48T

Anti-NFKB p52/NF-κB2 p100 细胞核因子/k基因结合核因子 p52/p100抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh IL-1R1/CD121a/IL1 Receptor I 白介素1受体1抗体 规格 0.1ml

LEP ELISA kit 大鼠瘦素 96T

Phospho-PLC gamma 1(Tyr775) 英文名称： 磷酸化磷酯酶Cγ1抗体 0.1ml

CD46 英文名称： 膜辅蛋白抗体 0.2ml

Anti-NFKB p52/NF-κB2 p100 细胞核因子/k基因结合核因子 p52/p100抗体Multi-class antibodies规格： 0.2ml

VEGFA Others Human 人 VEGF121b / VEGF-A 人细胞裂解液 (阳性对照)

人微内皮细胞RNAHCMEC miRNA5 μg

大额牛皮肤成纤维样细胞;BOS-1

PA319细胞，人细胞 人视网膜色素上皮细胞,D407细胞 人细胞;PC-3 [PC3]

Hep G2(人细胞) 5×106cells/瓶×2

ACY1 Others Mouse 小鼠 ACY1 / Aminoacylase-1 人细胞裂解液 (阳性对照)

CL-0395NCI-H2227(人小细胞细胞)5×106cells/瓶×2

CADM3 Others Rat 大鼠 CADM3 / NECL1 / IGSF4B 人细胞裂解液 (阳性对照)

小鼠肺平滑肌细胞完全培养基 100mL

NIH/3T3小鼠胚胎细胞 NIH/3T3 mouse embryo cells DMEM+10% FBS

IL36B Protein Human 重组人 IL36B / IL1F8 蛋白 (His 标签)

NCI-H2170(人肺鳞癌细胞) 5×106cells/瓶×2 HT-29(细胞)

组蛋白甲基转移酶SMYD3抗体说明书 CL-0394NCI-H2170(人肺鳞癌细胞)5×106cells/瓶×2

EFNB2 Others Rat 大鼠 Ephrin-B2 / EFNB2 人细胞裂解液 (阳性对照)

小鼠气管上皮细胞完全培养基 100mL

NIH/3T3小鼠胚胎细胞 NIH/3T3 mouse embryo cells DMEM+10%NBCS(小牛血清)

IL37 Protein Human 重组人 IL37 / IL1F7 / IL-1H4 蛋白

NCI-H226(人肺鳞癌细胞) 5×106cells/瓶×2 LoVo(细胞)